extract and purify造句
例句与造句
- studies on extracting and purifying edible pigment from natural annatto
天然红木食用色素的提取与纯化研究 - the method we used to extract and purify the water-soluble one was somewhat different from that we used in liposoluble pdne
其提取步骤和脂溶性物质pdne的提取分离稍有差别。 - the nuclei and nucleoli were extracted and purified and the sclerotia were induced from fuligo septica phaneroplasmodium, and then they were observed under tem
从煤绒菌显型原质团中提纯细胞核、核仁,诱导原质团形成菌核,并在透射电镜下观察。 - after the produre of extracting and purifying immunogen, the production of monoclonal antibodies was carried out . three balb / c mice, 6-week old, were used for immunization
首先选择六周龄的balb/c小鼠三只，按照免疫方案进行尾静脉和皮下的注射。 - the first part of our experiment is to extract and purify the total rna from the human liver tissue in the condition without rnase and to identify its integrity and purity
本研究第一部分包括肝组织中总rna的分离提取；rt-pcr扩增出血管抑素基因。首先，采集术后新鲜肝组织，并于无rnase的条件下分离纯化总rna。 - It's difficult to find extract and purify in a sentence. 用extract and purify造句挺难的
- the recombinant was screened by pcr, cleaved by bamhi, bglll to determine the director, and then was named pci-cea . after growing in large scale, plasmids were extracted and purified and quantified . 2
pciil2的构建：用oligo软件设计弓物，在上、下游引物的5’端加上xhol和sail酶切位点，以便于与载体连接。 - however, depending on the differences among e . coli strains, and the procedures used to extract and purify endotoxin that serves as standard for these measurements, some differences might occur in the specific activity
然而，依靠大肠杆菌张力的不同，用这些测量法的标准，过程中曾经析取和净化内毒素，一些不同的力量存在比活性。 - in this section i extract and purify out one concept : the function of this kind of newspaper is not merely give the magnanimity message to readers but to escort information receivers to contact with the information that they need
在应对策略中，提炼出一个观点:厂‘一播电子见j一仗节日表是信脚、的信窟、，其功能是作信启、的向导，而并不是仅仅展示海量信息。 - 1 . isolation, purification and characterization of lipovitellin from the ovary of female eriocheir sinensis broodstock lipovitellin was extracted and purified using gel chromatography and electrophoresis procedures from ovaries of chinese mitten-handed crab . one kind of lipovitellin was obtained
一、中华绒鳌蟹卵黄磷蛋白的分离、纯化与鉴定目前在判定虾蟹卵巢成熟和卵质时，大多采用性腺指数、蛋白质和脂类的组成与含量等生化指标，尚不够全面。 - a eukaryotic expression vector pcdna3.1-cptl was constructed by insert cp77 gene into the vector pcdna3.1 which is used in nucleic acid immunization . the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal . immu-nological reaction was induced by the expression of cptl after the vector entered into the mice body
本研究通过限制酶将cpti基因片段从载体pbluel3上切下，插入真核表达载体pcdna3.1，构建了用于核酸免疫的真核表达载体pcdna3.1-cpti；质粒大量提取和纯化后，通过肌肉注射的方法免疫balbc小鼠，基因表达产物刺激小鼠机体产生免疫反应，从而获得了抗cpti蛋白的抗体。 - however, animal organisms could not produce ifn commonly, many scholars had attempted to use virus infect animals in order to induce leukocyte to produce ifn . as a result, a very small quantity of ifn was produced and also the cost of production was very high, it was difficult to extract and purify ifn protein
但是，动物体内一般情况下不产生干扰素，国内外学者曾尝试利用病毒感染诱导动物机体白细胞产生干扰素，结果仅产生极其微量的ifn且生产成本高，难于提取纯化。 - the plasmids pci-mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl-1 blue, then the extracted and purified pci-mbl54 were used to transfect dhfr (-) chinese-hamster ovary ( cho ) cells . after screeened with g418 and cloned, 4 g418-resistent clones were randomly selected for detection of mrna expression by rt-pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鉴定、纯化重组质粒后，脂质体转染法将重组质粒导入中国仓鼠卵巢细胞(cho-dhfr~-)中，g418选择转染子并克隆化培养，经rt-pcr和分子灯塔探针杂交鉴定其mrna转录，获得4株稳定表达54位密码突变型mbl的cho细胞。 - with the development of dna recombination technology, there are also some reports about the expression of recombined ifn protein by prokaryotic expression system, but the expressed proteins often consist in the form of inclusion body = the operations about extracting and purifying proteins are very complicated, biological activity of proteins are not high
随着dna重组技术的发展，国内外亦有利用原核表达系统表达重组ifn蛋白的报道，但表达的目的蛋白常以包涵体形式存在，提取纯化操作步骤复杂、蛋白活性不高。